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Dynein-dynactin complex is essential for dendritic restriction of TM1-containing Drosophila Dscam

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  • معلومة اضافية
    • Contributors:
      Graduate School of Biomedical Sciences, Neuroscience Program; Lee Lab; Neurobiology; Shun-Jen (Jacob) S. Yang
    • الموضوع:
      2022
    • Collection:
      University of Massachusetts, Medical School: eScholarship@UMMS
    • نبذة مختصرة :
      Co-author Jacob S. Yang is a student in the Neuroscience program in the Graduate School of Biomedical Sciences (GSBS) at UMass Medical School. ; BACKGROUND: Many membrane proteins, including Drosophila Dscam, are enriched in dendrites or axons within neurons. However, little is known about how the differential distribution is established and maintained. METHODOLOGY/PRINCIPAL FINDINGS: Here we investigated the mechanisms underlying the dendritic targeting of Dscam[TM1]. Through forward genetic mosaic screens and by silencing specific genes via targeted RNAi, we found that several genes, encoding various components of the dynein-dynactin complex, are required for restricting Dscam[TM1] to the mushroom body dendrites. In contrast, compromising dynein/dynactin function did not affect dendritic targeting of two other dendritic markers, Nod and Rdl. Tracing newly synthesized Dscam[TM1] further revealed that compromising dynein/dynactin function did not affect the initial dendritic targeting of Dscam[TM1], but disrupted the maintenance of its restriction to dendrites. CONCLUSIONS/SIGNIFICANCE: The results of this study suggest multiple mechanisms of dendritic protein targeting. Notably, dynein-dynactin plays a role in excluding dendritic Dscam, but not Rdl, from axons by retrograde transport. ; Neuroscience
    • Relation:
      Link to Article in PubMed; PLoS One. 2008;3(10):e3504. Epub 2008 Oct 23. Link to article on publisher's site; 1932-6203 (Electronic); http://hdl.handle.net/20.500.14038/39264; https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=3065&context=oapubs&unstamped=1; https://escholarship.umassmed.edu/oapubs/2066; 1073598; oapubs/2066
    • الرقم المعرف:
      10.1371/journal.pone.0003504
    • Rights:
      Copyright: © 2008 Yang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
    • الرقم المعرف:
      edsbas.FEDE44D4