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Novel nanostructured biomaterials for biomedical applications

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  • معلومة اضافية
    • Contributors:
      Tarusha, Lorena; PAOLETTI, SERGIO
    • بيانات النشر:
      Università degli Studi di Trieste
    • الموضوع:
      2016
    • Collection:
      Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste)
    • نبذة مختصرة :
      The research activity reported in this thesis was focused on bottom-up design, production and characterization of biomaterials in the form of medical devices for biomedical applications. More specifically, both a biomaterial for anastomosis after colorectal cancer resection, and a biomaterial for chronic non-healing wounds have been designed and developed, by exploiting the same manufacturing strategy. In the first part of the work, the research has been mainly aimed to obtain a device for preventing the leakage of the anastomosis following surgical treatment of colorectal cancer. The anastomotic leakage is a defect of the intestinal wall at the anastomotic site, which leads to a communication between the intra- and extra-luminal compartments. This study was part of the scientific activity forecasted by the European project AnastomoSEAL (FP7, c.n.280929). The project aim was to develop a patch to be wrapped around the anastomosis, capable to promote the healing of the wound. Specific biomolecules were chosen as biomaterial components. Alginate was selected for its ability to form gels thus providing the physical matrix; Hyaluronic Acid (HA) was chosen for its ability to stimulate wound healing; Butyric acid (But) was chosen since recent data demonstrated its beneficial effect on colorectal anastomosis in animal models. The last two components have been also chemically combined in the Hyaluronic Acid Butyric ester (HABut) molecule. Patches with alginate and HA were produced by using various polymer concentrations, different alginate types (algal sources), and HA with different molecular weights, in order to fine-tune the composition and the performances for the final application. Moreover, in vitro biological tests were performed on the patch components (raw materials): the effects on cell viability, proliferation and extracellular matrix production were studied on primary human fibroblasts and on a normal-derived colonocyte cell line. Additional biological in vitro tests were conducted in order to study more in ...
    • Relation:
      http://hdl.handle.net/11368/2908088
    • Rights:
      info:eu-repo/semantics/openAccess
    • الرقم المعرف:
      edsbas.FD962023