Figure 7. Phylogenetic Analysis of FKF1/ZTL family members in plants. ; Residue identity at position 46 (Colored Bar) distinguishes ZTL-like, LKP2-like and FKF1-like proteins consistent with evolutionary diversification of signaling mechanisms. LKP2 is isolated to a clade containing Brassica rapa members that all contain a Q154L substitution. Al ZTL members contain G46 which is necessary to promote the alternative conformation of Q154. Spikemoss and liverwort FKF1’s are isolated indicating a possible intermediate function. Accession numbers for all sequences are shown after the protein name. The evolutionary history was inferred using the Minimum Evolution method (Rzhetsky and Nei, 1992). The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches (Felsenstein, 1985). The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method (Zuckerkandl and Pauling, 1965) and are in the units of the number of amino acid substitutions per site. The ME tree was searched using the Close-Neighbor-Interchange (CNI) algorithm (Nei and Kumar, 2000) at a search level of 1. The Neighbor-joining algorithm (Saitou and Nei, 1987) was used to generate the initial tree. The analysis involved 28 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 535 positions in the final dataset. Evolutionary analyses were conducted in MEGA7 (68). (Figure 7—figure supplement 1).

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