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RPE-1 GFP-H2B iCas9 cells undergoing paclitaxel-induced mitotic arrest

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Contributors:
      Jones, Mathew; Clarke, Paul; Bolderson, Emma
    • بيانات النشر:
      Zenodo
    • الموضوع:
      2022
    • Collection:
      Zenodo
    • نبذة مختصرة :
      Phasefocus LiveCyte video showing RPE-1 GFP-H2B iCas9 cells undergoing paclitaxel-induced mitotic arrest.Cells were treated with 1 μg/mL doxycycline for 96 hours to induce Cas9 expression and were imaged every 9 minutes for 48 hours at 20x magnification.From 3:17 cells start entering mitosis as indicated by their rounding up into a ball; from 12:44 mitotic cells begin to either slip from mitosis as indicated by rounded mitotic cells flattening or undergoing mitotic cell death as indicated by the presence of propidium iodide (red).
    • Relation:
      https://doi.org/10.5281/zenodo.6378317; https://doi.org/10.5281/zenodo.6378318; oai:zenodo.org:6378318
    • الرقم المعرف:
      10.5281/zenodo.6378318
    • الدخول الالكتروني :
      https://doi.org/10.5281/zenodo.6378318
    • Rights:
      info:eu-repo/semantics/openAccess ; Creative Commons Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/legalcode
    • الرقم المعرف:
      edsbas.F7E2B454