Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Searching for links between environmental and clinical mecA+Staphylococcus aureus: A comparative genomics study

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Contributors:
      European Commission; Foundation for Science and Technology; Fundação para a Ciência e a Tecnologia (Portugal); Rocha, Jaqueline; Silva, Vanessa; Poeta, Patrícia; Botelho, João; Manaia, Célia M.
    • بيانات النشر:
      Elsevier BV
    • الموضوع:
      2023
    • Collection:
      Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council)
    • نبذة مختصرة :
      9 Pág. ; Staphylococcus aureus integrate the list of highly virulent and antibiotic resistant pathogens, mainly due to the mecA gene, associated with methicillin resistance. Given the ubiquity of this species, the aim of this study was to investigate whether closely related mecA+S. aureus found in the environment can be also thrive as clinical isolates and if the respective accessory genome may suggest bacterial adaptation. The genomes of environmental (water, animal facilities, food products, n = 111) isolates were compared with closely related genomes of clinical origin (human patients, n = 103). These genomes, available in the public database NCBI, were analysed for phylogeny, accessory genome, and presence of selected clinically relevant genes (n = 104). The genomes of environmental isolates belonged to 18 multi-locus sequence types (MLSTs), 11 of which also included clinical genomes, a result confirmed based on core-genome analysis. Genes significantly (p ≤ 0.05) more frequent among environmental genomes were related with resistance to β-lactams (blaI, blaPCI), aminoglycosides (ant(6)-Ia), macrolides (mph(C), erm(B)), enterotoxins (seg, sei, sem, sen, seo, seu) and serine protease functions (splB), among others. Genes significantly more frequent among clinical genomes were associated with resistance to macrolides (erm(C)), phenicols (fexA), fosfomycin (murA), the leucocidin virulence gene (lukS-PV), and serine protease functions (splA, splE). It is suggested that mecA+S. aureus can be exchanged between clinical and environmental settings, with accessory traits (particularly antibiotic resistance, virulence and stress response) possibly being associated with the habitat. The interplay between phylogeny and accessory genome is an interesting contribution to better understanding the ecology and evolution of mecA+S. aureus. ; This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic ...
    • File Description:
      application/pdf
    • ISSN:
      0048-9697
      1879-1026
    • Relation:
      #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/EC//PTDC/SAUINF/30101/2017; info:eu-repo/grantAgreement/EC//PTDC/CTA-AMB/28196/2017; Centro de Biotecnología y Genómica de Plantas (CBGP); Publisher's version; https://doi.org/10.1016/j.scitotenv.2023.165078; Sí; Science of the Total Environment 895: e165078 (2023); http://hdl.handle.net/10261/332329; http://dx.doi.org/10.13039/501100001871; http://dx.doi.org/10.13039/501100000780; 2-s2.0-85163486022; https://api.elsevier.com/content/abstract/scopus_id/85163486022
    • الرقم المعرف:
      10.1016/j.scitotenv.2023.165078
    • الرقم المعرف:
      10.13039/501100001871
    • الرقم المعرف:
      10.13039/501100000780
    • Rights:
      open
    • الرقم المعرف:
      edsbas.F1E91854