Structural modeling of CRMPs.

(A) Homodimer modeling for (1) CRMPA and (2) CRMPB result in overlay of proteins. The PAE scores show good relative prediction for single chains but indicate that both chains do not interact, despite the high IDDT values. Sequence alignment blots indicate the number of sequences that could be aligned to each position. ( B) Multimer heterodimer prediction of PbCRMP1/3, PbCRMP1/4, PbCRMP2/3, and PbCRMP2/4. CRMPA clade members are colored light blue; CRMPB clade members are colored orange. PAE scores indicate that PbCRMP1/3 and PbCRMP2/4 do interact and that PbCRMP1/4 and PbCRMP2/3 do not interact. PbCRMP3 and PbCRMP4 are predicted in two fragments with an unstructured part (indicated by the low local pLDDT score). ( C) Prediction of heterodimer formation of CRMPA and CRMPB transmembrane domains after induction of RH-CRMPA-3HA floxed resulting in loss of the C-terminus and the last 2 transmembrane domains. New C-terminus is indicated in red. Notice the change in PAE score compared to full-length TMs shown on the right. Input sequences are in S2 Table , Structure B-H in S1 File . (TIF)