Modification of cardiac morphology was associated with impaired myocardial sensitivity to ischemia-reperfusion injury in a diet-induced metabolic syndrome model

International audience ; Background and aims: Metabolic Syndrome (MetS) is defined by multiple risk factors that predict type 2 diabetes and cardiovascular complications, such as myocardial infarction, especially in women. Consequently the aim of this study was to investigate in vivo and ex vivo the effects of a high-fat-high-sucrose diet (HFHSD) on the development of metabolic syndrome (MetS), cardiac morphology and sensitivity to ischemia-reperfusion injury of female Wistar rat.Materials and methods: Female Wistar rats, subjected to HFHSD (FHFD) or Normal Diet (FND) during 5 months, were explored in vivo every month with multimodal cardiovascular magnetic resonance (CMR). Cine-MRI (Magnetic Resonance Imaging) and arterial spin labeling (ASL-FAIR) techniques were used to determine cardiac morphology, function and perfusion. Triglyceride (TG) content in heart and liver 1was also evaluated with H Magnetic Resonance Spectroscopy (MRS). 1Sub-cutaneous and visceral adipose tissues were measured with H MRI. Then, rats underwent an intraperitoneal glucose tolerance test (IPGTT) to determine glycemic status. Finally, isolated heart were perfused with a physiological buffer containing 0.4 mM palmitate for 24 minutes before switching to 1.2 mM palmitate during 32 minutes low-flow (0.5 mL/min/g wet wt) ischemia. Next, flow was restored with 0.4 mM palmitate buffer for 32 minutes. High-energy phosphates and intracellular pH were measured during the experimental course by P magnetic resonance spectroscopy with simultaneous measurement of contractile function. Coronary flow was measured before and after ischemia. At the end of experiments, hearts were freeze-clamped for biochemical assays. Results: In FHFD vs. FND, CMR showed an increase of systolic wall thickness over time (p<0.05) and diastolic wall thickness at 3 and 5 months 1(p<0.01); H MRS showed that hepatic TG content was increased (p<0.01) at 5 month but myocardial TG content was not different. IPGTT showed a significant glucose intolerance (p<0.001) and ...