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Collagenase treatment appears to improve cartilage tissue integration but damage to collagen networks is likely permanent

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  • معلومة اضافية
    • بيانات النشر:
      SAGE Publications Ltd
    • الموضوع:
      2022
    • Collection:
      Queensland University of Technology: QUT ePrints
    • نبذة مختصرة :
      When repairing cartilage defects a major challenge is achieving high-quality integration between the repair tissue and adjacent native cartilage. Matrix-rich cartilage is not easily remodeled, motivating several studies to trial enzyme treatment of the tissue interface to facilitate remodeling and integration. Studying and optimizing such processes is tedious, as well as potentially expensive, and thus simpler models are needed to evaluate the merits of enzyme treatment on cartilage tissue integration. Herein, we used engineered cartilage microtissues formed from bone marrow-derived stromal cells (BMSC) or expanded articular chondrocytes (ACh) to study the impact of enzyme treatment on cartilage tissue integration and matrix remodeling. A 5-min treatment with collagenase appeared to improve cartilage microtissue integration, while up to 48 h treatment with hyaluronidase did not. Alcian blue and anti-collagen II staining suggested that collagenase treatment did facilitate near seamless integration of cartilage microtissues. Microtissue sections were stained with Picrosirius red and characterized using polarized light microscopy, revealing that individual microtissues contained a collagen network organized in concentric shells. While collagenase treatment appeared to improve tissue integration, assessment of the collagen fibers with polarized light indicated that enzymatically damaged networks were not remodeled nor restored during subsequent culture. This model and these data paradoxically suggest that collagen network disruption is required to improve cartilage tissue integration, but that the disrupted collagen networks are unlikely to subsequently be restored. Future studies should attempt to limit collagen network disruption to the surface of the cartilage, and we recommend using Picrosirius red staining and polarized light to assess the quality of matrix remodeling and integration.
    • File Description:
      application/pdf
    • Relation:
      https://eprints.qut.edu.au/231293/1/110266864.pdf; Shajib, Md Shafiullah, Futrega, Kathryn, Jacob Klein, Travis, Crawford, Ross W., & Doran, Michael Robert (2022) Collagenase treatment appears to improve cartilage tissue integration but damage to collagen networks is likely permanent. Journal of Tissue Engineering, 13.; http://purl.org/au-research/grants/nhmrc/1130013; http://purl.org/au-research/grants/nhmrc/1083857; https://eprints.qut.edu.au/231293/; Centre for Biomedical Technologies; Faculty of Engineering; School of Mechanical, Medical & Process Engineering; Faculty of Health; School of Biomedical Sciences
    • الدخول الالكتروني :
      https://eprints.qut.edu.au/231293/
    • Rights:
      free_to_read ; http://creativecommons.org/licenses/by-nc/4.0/ ; The Author(s) 2022 ; This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au
    • الرقم المعرف:
      edsbas.EC0940E2