Figure 1. Experimental design for evaluation of early effects of mild drought treatment on Brassica rapa R500 plants and soil water potential during the experiment. ; (A) Schematic illustration of the experimental design with days of the experiment and collection/measurement times. Top: The time of the experiment in days after sowing (DAS) is plotted with the orange shade representing the days of mild drought treatment starting at the end of 15 DAS. Center: Purple shade (between 18 and 20 DAS) indicates the 2 days of continuous collection/measurements. The x-axis represents the 48 hr of the experiment in Zeitgeber Time (ZT0 = lights on in the growth chambers). The dotted yellow lines represent the ZT times for collection and measurements of both well-watered and droughted plants. Bottom: White and black bars represent the photoperiod in the growth chambers (14 hr/10 hr; Day/Night) for Days 3 and 4. (B) Soil water potential (Ψs) progression for well-watered (solid symbols) and mild droughted (open symbols) B. rapa R500 plants (n = at least 6) after the start of the drought treatment during the physiology (triangles) and RNA-seq (circles) time-course experiments.

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