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Capillary electrophoresis analysis of EUA-kit N1 NTC reaction detecting oligonucleotide peaks with median size of 20 bp (non-reacted primers and probe) and 55 bp (putative homo- and hetero-duplexes).

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  • معلومة اضافية
    • الموضوع:
      2021
    • Collection:
      Smithsonian Institution: Digital Repository
    • نبذة مختصرة :
      Additional minor peaks at 42 bp and 51 bp were also present. Oligonucleotides with calculated free energy (ΔG) are predicted for low energy interaction < -3.0 kcal∙mole-1 and duplex formation <8.9 kcal∙mole-1. Maximum free energy is based on 100% complementary sequence. The number of theoretical pairs favorable for duplex formation are listed on right side of chart. (DOCX)
    • Relation:
      https://figshare.com/articles/journal_contribution/Capillary_electrophoresis_analysis_of_EUA-kit_N1_NTC_reaction_detecting_oligonucleotide_peaks_with_median_size_of_20_bp_non-reacted_primers_and_probe_and_55_bp_putative_homo-_and_hetero-duplexes_/17207668
    • الرقم المعرف:
      10.1371/journal.pone.0260487.s002
    • Rights:
      CC BY 4.0
    • الرقم المعرف:
      edsbas.E5A4767C