Contributors: Comenius University in Bratislava; Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz; University of Lódź = Uniwersytet Łódzki; National Academy of Sciences of Belarus (NASB); Laboratoire de chimie de coordination (LCC); Institut de Chimie de Toulouse (ICT); Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3); Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP); Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3); Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS); Slovak Research and Development Agency; State Committee on Science and Technology of the Republic of Belarus; NAWA International Academic Partnership Programme EUROPARTNER; Scientific Grant Agency of the Ministry of Education, Science, Research, and Sport of the Slovak Republic; Cultural and Educational Grant Agency of the Ministry of Education, Science, Research, and Sport of the Slovak Republic (KEGA)
نبذة مختصرة : Supplementary Materials: The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/pharmaceutics14081596/s1. Table S1: Three-way ANOVA results on viscosities of amphiphilic phosphorous dendrons of the first (D1) and second (D2) generation: matching by factors: concentration & dendron generation; Table S2: Three-way ANOVA results on viscosities of amphiphilic phosphorous dendrons of the first (D1) and second (D2) generation: matching by factors: concentration & dendron generation ; International audience ; Drug delivery by dendron-based nanoparticles is widely studied due to their ability to encapsulate or bind different ligands. For medical purposes, it is necessary (even if not sufficient) for these nanostructures to be compatible with blood. We studied the interaction of amphiphilic dendrons with blood samples from healthy volunteers using standard laboratory methods and rheological measurements. We did not observe clinically relevant abnormalities, but we found a concentration-dependent increase in whole blood viscosity, higher in males, presumably due to the formation of aggregates. To characterize the nature of the interactions among blood components and dendrons, we performed experiments on the liposomes and exosomes as models of biological membranes. Based on results obtained using diverse biophysical methods, we conclude that the interactions were of electrostatic nature. Overall, we have confirmed a concentration-dependent effect of dendrons on membrane systems, while the effect of generation was ambiguous. At higher dendron concentrations, the structure of membranes became disturbed, and membranes were prone to forming bigger aggregates, as visualized by SEM. This might have implications for blood flow disturbances when used in vivo. We propose to introduce blood viscosity measurements in early stages of investigation as they can help to optimize drug-like properties of potential drug carriers.
Processing Request
No Comments.