نبذة مختصرة : Enzimska sinteza biodizela provedena je u kotlastom reaktoru uz katalizator Amano lipazu podrijetlom iz Burkholderia cepacia pri dva različita pH reakcijske smjese, pH 10,0 i pH 7,0 i temperaturu od 50°C. Udio metilnih estera masnih kiselina u biodizelu kretao se u rasponu od 97,3 do 97,6 %, a udio slobodnog glicerola u rasponu od 0,043 do 0,144 % (m/m). Pročišćavanje biodizela provedeno je konvencionalnim pročišćavanjem uparavanjem, sušenjem i centrifugiranjem, i primjenom membranske ultrafiltracije s ciljem uklanjanja zaostalog glicerola. Nakon konvencionalnog pročišćavanja biodizela udio glicerola je bio ispod razine detekcije u oba uzorka sintetiziranog biodizela. Za pročišćavanje na filtracijskom modulu Amicon Stirred Cell korištene su celulozne i polietersulfonske ultrafiltracijske membrane veličine pora 10 kDa, promjera 44,5 mm uz tlak od 2 do 4 bara, sobnoj temperaturi i temperaturi od 45 °C uz dodatak vode od 0,1 % (m/m) i 0,2 % (m/m). Primjenom celulozne i polietersulfonske ultrafiltracijske membrane u opisanim uvjetima membranske filtracije na modulu Amicon Stirred Cell nije došlo do uspješne filtracije sintetiziranog biodizela. ; The enzymatic synthesis of biodiesel was carried out in a batch bioreactor with an Amano lipase from Burkholderia cepacia at two different pH of the reaction mixture, pH 10,0 and pH 7,0 and a temperature of . 50 ° C. The content of fatty acid methyl esters in biodiesel ranged from 97.3 to 97.6% and the free glycerol content ranged from 0.043 to 0.144% (m / m). To remove residual glycerol purification was carried out by conventional methods by evaporation, drying and centrifugation, and the application of membrane ultrafiltration. After conventional biodiesel purification, the glycerol content was below the detection level in both samples of the synthesized biodiesel. For purification on the Amicon Stirred Cell filtration module, cellulose ultrafiltration membranes with a pore size of 10 kDa, 44.5 mm in diameter at 4 bar pressure, room temperature and 45 ° C were added with ...
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