Contributors: Mastropasqua, L.; Toto, L.; Chiricosta, L.; Diomede, F.; Gugliandolo, A.; Silvestro, S.; Marconi, G. D.; Sinjari, B.; Vecchiet, J.; Cipollone, F.; D'Ardes, D.; Auricchio, A.; Lanzini, M.; Caputi, S.; D'Aloisio, R.; Mazzon, E.; Trubiani, O.
نبذة مختصرة : Recent studies have focused their attention on conjunctivitis as one of the symptoms of coronavirus disease 2019 (COVID-19). Therefore, tear samples were taken from COVID-19 patients and the presence of SARS-CoV-2 was evidenced using Real Time reverse transcription polymerase chain reaction. The main aim of this study was to analyze mRNA expression in the tears of patients with COVID-19 compared with healthy subjects using Next Generation Sequencing (NGS). The functional evaluation of the transcriptome highlighted 25 genes that differ statistically between healthy individuals and patients affected by COVID-19. In particular, the NGS analysis identified the presence of several genes involved in B cell signaling and keratinization. In particular, the genes involved in B cell signaling were downregulated in the tears of COVID-19 patients, while those involved in keratinization were upregulated. The results indicated that SARS-CoV-2 may induce a process of ocular keratinization and a defective B cell response.
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