Ultrasound-assisted extraction of bioactive compounds from algae Pelvetia canaliculata from the Atlantic Ocean into sunflower oil ; Ultrazvočna ekstrakcija bioaktivnih snovi iz alg vrste Pelvetia canaliculata iz Atlantskega oceana v sončnično olje

The aim of this master thesis was to test antioxidants from algae Pelvetia canaliculata L. that might offer healthier alternative to synthetic antioxidants. In this study, lyophilized P. canaliculata L. from the Portuguese coast was added directly to the antioxidant free sunflower oil. Ultrasound Assisted Extraction for extracting bioactive compounds from the algae into the oil included the following optimization parameters: algae concentration and mass and extraction time. After extraction, the following parameters were analysed in the oils: oxidation products, acidity, colour and determination of chromatic characteristics (chromatic coordinates (x,y), transparency, dominant wavelength and purity), chlorophyll pigments, carotenoids, flavonoids, total phenolic content, antioxidant activity by DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) assays, and sensory analysis. Bioactive pigments were successfully extracted from P. canaliculata into the sunflower oil, which consequently increased oil’s antioxidant activity. The chlorophyll and carotenoid content in the supplemented sunflower oils increased as much as 330 and 8-times, respectively. However, the flavonoid and phenolic extractions were limited, with extraction yields up to only 9.40% and 3.10%, respectively. The addition of P. canaliculata did not affect the chemical quality parameters (acidity and oxidation products) of the oil, however it affected the physical and sensory characteristics (colour, smell). In this study, we confirmed that such oils can be beneficial for the food industry, as they have an increased shelf life due to the extracted natural compounds, which also present some health benefits. ; Namen magistrskega dela je bil preveriti učinkovitost antioksidantov iz alge Pelvetia canaliculata L., ki lahko predstvaljajo bolj zdravo alternativo sintetičnim antioksidantom, ki imajo lahko negativne učinke na zdravje. V tej študiji smo liofilizirane alge P. canaliculata s portugalske obale dodali neposredno ...