Study of the tolerance of high doses of drugs for external use based on fipronil, praziquantel, moxidectin and pyriproxyfen

The purpose of the research is to study the tolerance of increased doses of topical preparations based on fipronil, praziquantel, moxidectin and pyriproxyfen in dogs and cats at increased therapeutic doses.Materials and methods. In the laboratory of ectoparasitoses on the basis of VNIIP, a branch of the Federal State Budget Scientific Institution of the Federal Scientific Center of VIEV RAS, two preparations were developed in the form of a solution for external use for dogs and cats based on praziquantel, pyriproxyfen, imidacloprid and moxidectin. Work on the study of tolerance to high doses was carried out on 15 kittens and 15 puppies under the age of one year, 15 adult cats and 15 adult dogs. Animals were divided into 8 experimental and 4 control groups according to the principle of analogues, 5 animals each. The drugs were used at 1.5 and 2 times the increased therapeutic dose four times with an interval of 7 days. The animals were observed for 30 days; noted the general condition, behavior, appetite, controlled weight and body temperature. Before the start of the experiment and 30 days after the start of the use of the drugs, blood was taken for general clinical and biochemical studies.Results and discussion. It was found that drugs in 1.5 and 2 times increased therapeutic doses, applied four times with an interval of 7 days, did not have a negative effect on carnivores. There were no changes in behavior, body temperature and weight, physical condition, clinical status, hematological and biochemical parameters of blood and functions of internal organs. The drug was well tolerated by dogs and cats of different breeds of different age groups, as well as by puppies and kittens. Manifestations of any allergic reactions were not observed. The obtained results of the studies confirm the safety of the use of drugs in practice in the recommended dosing regimen.