Shedding light on human brain activity - Biomedical signal processing of changes in tissue oxygenation and hemodynamics measured non-invasively using functional near-infrared spectroscopy: new methods and applications

Light can be used to measure the activity of the human brain non-invasively. This is realized by shining near-infrared light into brain tissue, measuring the diffuse reflected light at different wavelengths, and determining the concentration changes of oxy- and deoxyhemoglobin ([O2Hb], [HHb]) which are related to changes in tissue hemodynamics and oxygenation – and thus to brain activity. This method, termed ‘functional near-infrared spectroscopy’ (fNIRS), is increasingly employed for basic brain research, and its routine usage for medical applications in a clinical setting is imminent. The thesis set out to address three aims: develop and apply new approaches in order to (i) improve the fNIRS signal quality; (ii) realize advanced multivariate signal-analysis in the time-frequency domain using fNIRS signals; and (iii) investigate the systemic confounders in fNIRS studies by studying the effect of changes in partial pressure of arterial CO2 (PaCO2) on fNIRS-derived changes in brain hemodynamics and oxygenation. The first aim was tackled by developing two novel signal processing methods that detect and remove movement artifacts (MAs) from fNIRS signals, either by using only the signal characteristic of the fNIRS input signal by itself (the ‘movement artifact removal algorithm’, MARA) or by adding signals from an accelerometer (the ‘acceleration-based movement artifact reduction algorithm’, AMARA). Both algorithms were successfully validated. Another study investigated how different methods for determining [O2Hb] and [HHb] are affected by MAs. A systematic analysis was performed showing that multi-distance based fNIRS methods are superior to single-distance ones with regard to their robustness to MAs. In another work, a general equation was derived (based on measured data) for modeling the light-path length in human brain tissue (i.e. the differential pathlength factor, DPF) depending on wavelength and age of the subject. The equation can be used in all fNIRS applications where the light transport through tissue is ...