Contributors: Federal University of Rio de Janeiro; Department of Biochemistry, University of Cambridge; University of Cagliari; Institut des Biomolécules Max Mousseron Pôle Chimie Balard (IBMM); Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS); Imperial College London; University of Cambridge UK (CAM); This research was funded by Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estadodo Rio de Janeiro (FAPERJ; E-26/110.111/2014 for F.L.C.S. and T.E.; E-026/203.254/2017 for T.E.),Isaac Newton Trust research grant for T.E., Global Challenge Research Fund (ref. 102642/A19819)for T.E., A.G.T., and G.J.B., and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–CAPES-Brasil (Finance code 001), Brazil. L.B. and D.B.M. were recipients of CAPES scholarships andC.S.F. was recipient of the CNPq scholarship (grant GM). The APC was covered partially by FAPERJ,PROEX funding via the post-graduate course in Nutrition, and the University of Cambridge.
نبذة مختصرة : International audience ; The rise in prevalence of obesity in women of reproductive age in developed and developing countries might propagate intergenerational cycles of detrimental effects on metabolic health. Placental lipid metabolism is disrupted by maternal obesity, which possibly affects the life-long health of the offspring. Here, we investigated placental lipid metabolism in women with pre-gestational obesity as a sole pregnancy complication and compared it to placental responses of lean women. Open profile and targeted lipidomics were used to assess placental lipids and oxidised products of docosahexaenoic (DHA) and arachidonic acid (AA), respectively, neuroprostanes and isoprostanes. Despite no overall signs of lipid accumulation, DHA and AA levels in placentas from obese women were, respectively, 2.2 and 2.5 times higher than those from lean women. Additionally, a 2-fold increase in DHA-derived neuroprostanes and a 1.7-fold increase in AA-derived isoprostanes were seen in the obese group. These changes correlated with a 70% decrease in placental FABP1 protein. Multivariate analyses suggested that neuroprostanes and isoprostanes are associated with maternal and placental inflammation and with birth weight. These results might shed light on the molecular mechanisms associated with altered placental fatty acid metabolism in maternal pre-gestational obesity, placing these oxidised fatty acids as novel mediators of placental function.
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