Identification of genetic abnormalities in nasopharyngeal carcinoma by comparative genomic hybridization and interphrase cytogenetics.

Fan Chung-sze. ; Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. ; Includes bibliographical references. ; Abstracts in English and Chinese. ; Acknowledgements --- p.i ; Abstract --- p.ii ; List of Tables --- p.vii ; List of Figures --- p.viii ; List of Abbreviations --- p.x ; Table of Contents --- p.xi ; Chapter Chapter 1 --- Introduction ; Chapter 1.1 --- Nasopharyngeal Carcinoma --- p.1-1 ; Chapter 1.1.1 --- Histology of NPC --- p.1-1 ; Chapter 1.1.2 --- Etiological Factors --- p.1-2 ; Chapter 1.1.3 --- Genetic Changes in NPC --- p.1-5 ; Chapter 1.2 --- Background of Present Study --- p.1-14 ; Chapter 1.3 --- Aims of Study --- p.1-15 ; Chapter Chapter 2 --- Comparative Genomic Hybridization and Fluorescence In- Situ Hybridization ; Chapter 2.1 --- Introduction --- p.2-1 ; Chapter 2.2 --- Fluorescence In-Situ Hybridization (FISH) --- p.2-2 ; Chapter 2.2.1 --- Principle of FISH --- p.2-2 ; Chapter 2.2.2 --- Applications of FISH --- p.2-2 ; Chapter 2.2.3 --- Advantages and Limitations --- p.2-5 ; Chapter 2.3 --- Comparative Genomic Hybridization (CGH) --- p.2-7 ; Chapter 2.3.1 --- Principle of CGH --- p.2-7 ; Chapter 2.3.2 --- Applications of CGH --- p.2-8 ; Chapter 2.3.3 --- Advantages and Limitations --- p.2-10 ; Chapter 2.4 --- Method of CGH --- p.2-13 ; Chapter 2.4.1 --- CGH Probe Preparation --- p.2-13 ; Chapter 2.4.2 --- CGH Template Preparation --- p.2-21 ; Chapter 2.4.3 --- Hybridization --- p.2-23 ; Chapter 2.4.4 --- Post-hybridization --- p.2-23 ; Chapter 2.4.5 --- Fluorescence Detection --- p.2-24 ; Chapter 2.4.6 --- Image Acquisition and Analysis --- p.2-24 ; Chapter 2.5 --- Method of Interphase FISH --- p.2-29 ; Chapter 2.5.1 --- FISH Probe Preparation --- p.2-29 ; Chapter 2.5.2 --- FISH Template Preparation --- p.2-29 ; Chapter 2.5.3 --- Hybridization --- p.2-30 ; Chapter 2.5.4 --- Post-hybridization --- p.2-30 ; Chapter 2.5.5 --- Fluorescence Detection --- p.2-30 ; Chapter 2.5.6 --- Scoring of FISH Signals --- p.2-31 ; Chapter 2.5.7 --- Threshold Determination --- p.2-31 ; Chapter Chapter ...