Contributors: Biologie des Spirochètes / Biology of Spirochetes; Université Paris Cité (UPCité)-Microbiologie Intégrative et Moléculaire (UMR6047); Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Centre National de Référence de la Leptospirose - National Reference Center Leptospirosis (CNR); Institut Pasteur Paris (IP)-Université Paris Cité (UPCité); VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS); Eurofins Biomnis – Biologie Médicale Spécialisée Lyon (EB-BMS); Laboratoire CERBA Saint Ouen l'Aumône; This study was supported and financed by the Pasteur Institute of Paris and the Veterinary Analysis Laboratory (LAV) at VetAgroSup, Lyon, France.; We would like to thank the sampling department of Santé Pulique France for providing the laboratory with samples from hospitals. We thank the staff of the Reference Center for Leptospirosis at the Pasteur Institute, Farida Zinini, Jean-François Mariet and Vallier Sordoillet, for processing the human samples. We also thank the Laboratoire des analyses vétérinaires (LAV) at VetAgro Sup, and Marine Le Guyader, Elisa Boissy and Angeli Kodjo in particular, for providing the samples from dogs.
نبذة مختصرة : International audience ; Leptospirosis is a bacterial zoonotic disease. Humans and dogs are susceptible hosts, with similar clinical manifestations ranging from a febrile phase to multiple organ dysfunction. The incidence of leptospirosis in mainland France is relatively high, at about 1 case per 100,000 inhabitants, but our knowledge of the strains circulating in humans and dogs remains limited. We studied the polymorphism of the lfb1 gene sequences in an exhaustive database, to facilitate the identification of Leptospira strains. We identified 46 species-groups (SG) encompassing the eight pathogenic species of Leptospira . We sequenced the lfb1 gene amplification products from 170 biological samples collected from 2019 to 2021: 110 from humans and 60 from dogs. Epidemiological data, including vaccination status in dogs, were also collected. Three Leptospira species displaying considerable diversity were identified: L. interrogans , with eight lfb1 species-groups (including five new lfb1 species-groups) in humans and dogs; L. kirschneri , with two lfb1 species-groups in humans and dogs; and L. borgpetersenii , with one lfb1 species-group in humans only. The lfb1 species-group L. interrogans SG1, corresponding to serovar Icterohaemorrhagiae or Copenhageni, was frequently retrieved from both humans and dogs ( n= 67/110; 60.9% and n= 59/60; 98.3% respectively). A high proportion of the affected dogs developed the disease despite vaccination ( n= 30/60; 50%). Genotyping with the polymorphic lfb1 gene is both robust and simple. This approach provided the first global picture of the Leptospira strains responsible for acute infections in mainland France, based on biological samples but without the need for culture. Identification of the Leptospira strains circulating and their changes over time will facilitate more precise epidemiological monitoring of susceptible and reservoir species. It should also facilitate the monitoring of environmental contamination, making it possible to implement preventive measures and to ...
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