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Comprehensive proteomic analysis reveals omega-3 fatty acids to counteract endotoxin-stimulated metabolic dysregulation in porcine enterocytes

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  • معلومة اضافية
    • Contributors:
      T.S. Sundaram; M.F. Addi; C. Giromini; R. Rebucci; S. Pisanu; D. Pagnozzi &; A. Baldi
    • بيانات النشر:
      Springer Nature Publishing Group
    • الموضوع:
      2023
    • Collection:
      The University of Milan: Archivio Istituzionale della Ricerca (AIR)
    • نبذة مختصرة :
      Omega-3 polyunsaturated fatty acids (n-3 PUFA), such as the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are reported to beneficially affect the intestinal immunity. The biological pathways modulated by n-3 PUFA during an infection, at the level of intestinal epithelial barrier remain elusive. To address this gap, we investigated the proteomic changes induced by n-3 PUFA in porcine enterocyte cell line (IPEC-J2), in the presence and absence of lipopolysaccharide (LPS) stress conditions using shotgun proteomics analysis integrated with RNA-sequencing technology. A total of 33, 85, and 88 differentially abundant proteins (DAPs) were identified in cells exposed to n-3 PUFA (DHA:EPA), LPS, and n-3 PUFA treatment followed by LPS stimulation, respectively. Functional annotation and pathway analysis of DAPs revealed the modulation of central carbon metabolism, including the glycolysis/gluconeogenesis, pentose phosphate pathway, and oxidative phosphorylation processes. Specifically, LPS caused metabolic dysregulation in enterocytes, which was abated upon prior treatment with n-3 PUFA. Besides, n-3 PUFA supplementation facilitated enterocyte development and lipid homeostasis. Altogether, this work for the first time comprehensively described the biological pathways regulated by n-3 PUFA in enterocytes, particularly during endotoxin-stimulated metabolic dysregulation. Additionally, this study may provide nutritional biomarkers in monitoring the intestinal health of human and animals on n-3 PUFA-based diets.
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/38062040; info:eu-repo/semantics/altIdentifier/wos/WOS:001142614900036; volume:13; firstpage:1; lastpage:15; numberofpages:15; journal:SCIENTIFIC REPORTS; https://hdl.handle.net/2434/1074692; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85178884183; https://www.nature.com/articles/s41598-023-48018-3#citeas
    • الرقم المعرف:
      10.1038/s41598-023-48018-3
    • الدخول الالكتروني :
      https://hdl.handle.net/2434/1074692
      https://doi.org/10.1038/s41598-023-48018-3
      https://www.nature.com/articles/s41598-023-48018-3#citeas
    • Rights:
      info:eu-repo/semantics/openAccess
    • الرقم المعرف:
      edsbas.D09B6B7