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Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality

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  • معلومة اضافية
    • بيانات النشر:
      MDPI
    • الموضوع:
      2014
    • Collection:
      DIGIBUG: Repositorio Institucional de la Universidad de Granada
    • نبذة مختصرة :
      Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.
    • ISSN:
      1420-3049
    • Relation:
      Giampieri, F.; et al. Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality. Molecules, 19(6): 7798-7816 (2014). [doi:10.3390/molecules19067798]; http://hdl.handle.net/10481/32890
    • الرقم المعرف:
      10.3390/molecules19067798
    • الدخول الالكتروني :
      http://hdl.handle.net/10481/32890
      https://doi.org/10.3390/molecules19067798
    • Rights:
      Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License ; http://creativecommons.org/licenses/by-nc-nd/3.0/ ; info:eu-repo/semantics/openAccess
    • الرقم المعرف:
      edsbas.CFBB9F8E