Changes in growth and production of non-psychotropic cannabinoids induced by pre-sowing treatment of hemp seeds with cold plasma, vacuum and electromagnetic field /

In this study, the effects of seed treatments with different stressors, such as cold plasma (CP), a vacuum and an electromagnetic field (EMF), on the in vitro germination of industrial hemp cv. Futura 75 were compared with the effects on germination in the field, plant growth, and the amount of major cannabinoids in the leaves and inflorescences of female plants. CP and EMF (but not vacuum) treatments improved in vitro seed germination, but had no impact on germination in the field. EMF treatment increased the weight of the above-ground part of male and female plants grown for 4 months by 65–70% and the number of female inflorescences by 70%. CP stimulated the growth of male plants (weight increased 1.4 times) but reduced the growth of female plants. Vacuum treatment did not induce changes in the growth of female and male plants. Vacuum and EMF treatments did not change the amount of cannabidiolic acid (CBDA), but CP decreased the CBDA content in hemp leaves by 41%. Vacuum treatment increased the amount of CBDA in female plant inflorescences by 26%. Thus, hemp seed treatment with EMF has a potential application for increasing the biomass of female plants. CP treatment can be used to increase male plant production while vacuum treatment can stimulate CBD production.