Contributors: Institut für Genomforschung und Systembiologie; Universität Bielefeld-Centrum für Biotechnologie; CLIB Graduate Cluster Industrial Biotechnology; Lehrstuhl für Mikrobiologie; Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg (FAU); Bioinformatics Resource Facility; AG Genominformatik; Universität Bielefeld-Technische Fakultät; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas; Universidade Federal de Minas Gerais = Federal University of Minas Gerais Belo Horizonte, Brazil (UFMG); Cellular and Molecular Parasitology / Parasitologia Celular e Molecular Belo Horizonte, Brésil; Fiocruz Minas - René Rachou Research Center / Instituto René Rachou Belo Horizonte, Brésil; Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ); Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ); Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP); Instituto de Ciências Biológicas; Federal University of Para - Universidade Federal do Pará - UFPA Belém, Brazil (UFPA); Prévention et Thérapie Moléculaires des Maladies Infectieuses Humaines; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS); Service de Microbiologie; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Faculté de Médecine-Hôpital Necker - Enfants Malades AP-HP; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP); Centre Hospitalier Universitaire de Rennes CHU Rennes = Rennes University Hospital Pontchaillou; Bielefeld University and the Ministry of Innovation, Science, Research and Technology of North Rhine-Westphalia. LO and AB were supported by the Deutsche Forschungsgemeinschaft in frame of SFB 796 (project B5).
نبذة مختصرة : International audience ; BACKGROUND: Corynebacterium pseudotuberculosis is generally regarded as an important animal pathogen that rarely infects humans. Clinical strains are occasionally recovered from human cases of lymphadenitis, such as C. pseudotuberculosis FRC41 that was isolated from the inguinal lymph node of a 12-year-old girl with necrotizing lymphadenitis. To detect potential virulence factors and corresponding gene-regulatory networks in this human isolate, the genome sequence of C. pseudotuberculosis FCR41 was determined by pyrosequencing and functionally annotated. RESULTS: Sequencing and assembly of the C. pseudotuberculosis FRC41 genome yielded a circular chromosome with a size of 2,337,913 bp and a mean G+C content of 52.2%. Specific gene sets associated with iron and zinc homeostasis were detected among the 2,110 predicted protein-coding regions and integrated into a gene-regulatory network that is linked with both the central metabolism and the oxidative stress response of FRC41. Two gene clusters encode proteins involved in the sortase-mediated polymerization of adhesive pili that can probably mediate the adherence to host tissue to facilitate additional ligand-receptor interactions and the delivery of virulence factors. The prominent virulence factors phospholipase D (Pld) and corynebacterial protease CP40 are encoded in the genome of this human isolate. The genome annotation revealed additional serine proteases, neuraminidase H, nitric oxide reductase, an invasion-associated protein, and acyl-CoA carboxylase subunits involved in mycolic acid biosynthesis as potential virulence factors. The cAMP-sensing transcription regulator GlxR plays a key role in controlling the expression of several genes contributing to virulence. CONCLUSION: The functional data deduced from the genome sequencing and the extended knowledge of virulence factors indicate that the human isolate C. pseudotuberculosis FRC41 is equipped with a distinct gene set promoting its survival under unfavorable environmental ...
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