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Ketoisophorone synthesis with an immobilized alcohol dehydrogenase

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  • معلومة اضافية
    • الموضوع:
      2019
    • Collection:
      Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB
    • نبذة مختصرة :
      Altres ajuts: Authors also thank COST Action CM 1303-Systems Biocatalysis for financial support. ; The monoterpenoid α-isophorone is sourced from the available and renewable plant dry matter, as well as a waste recovery operation from acetone. This compound, can be hydroxylated to 4-hydroxy-isophorone which is the main precursor for the synthesis of ketoisophorone. On its turn, ketoisophorone is a key intermediate for the production of carotenoids and Vitamin E. Here, the enzymatic oxidation of 4-hydroxy-isophorone to ketoisophorone is demonstrated employing an alcohol dehydrogenase (ADHaa) from Artemisia annua and a NADPH oxidase (NOX), as a cofactor regeneration enzyme. After 24 h of reaction and an initial substrate concentration of 50 mM, 95.7 % yield and a space time yield of 6.52 g Lâ»Â¹â€‰dayâ»Â¹ could be obtained. Furthermore, the immobilization of the alcohol dehydrogenase was studied on 17 different supports. An epoxy-functionalized agarose resulted in the highest metrics, 100±0% immobilization yield and 58.2±3.5 % retained activity. Finally, the immobilized ADHaa was successfully implemented in 4 reaction cycles (96 h operation) presenting a biocatalyst yield of 23.4 g product gâ»Â¹ of enzyme. It represents a 2.5-fold increase compared with the reaction with soluble enzymes.
    • File Description:
      application/pdf
    • ISSN:
      18673880
    • Relation:
      European Commission 635734; Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1462; CHEMCACHEM; Vol. 11, issue 19 (Oct. 2019), p. 4862-4870; https://ddd.uab.cat/record/214989; urn:10.1002/cctc.201901090; urn:oai:ddd.uab.cat:214989; urn:articleid:18673880v11p4862; urn:scopus_id:85074188781; urn:wos_id:000485534000001; urn:altmetric_id:66432379
    • الدخول الالكتروني :
      https://ddd.uab.cat/record/214989
    • Rights:
      open access ; Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets. ; https://rightsstatements.org/vocab/InC/1.0/
    • الرقم المعرف:
      edsbas.CC626E3D