Coxiella_ Verocells_28dpi

Coxiella burnetii Nine Mile phase II clone 4 (NMIIC4) strain. Coxiella cells purified from infected Vero cells. ; Tilt Series Date: 2017-09-25 Data Taken By: Debnath Ghosal Species / Specimen: Coxiella burnetii Strain: Nine Mile phase II clone 4 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -7.0 μm, magnification: 27500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: Strain sent to us by Robert Heinzen. Data collected and processed by Debnath Ghosal using Caltech tomography pipeline. Purification / Growth Conditions / Treatment: Refer to Heinzen lab. Sample Preparation: Freezing: 100% humidity, 4µl sample, Blot force 6, blot time 4 sec. Wait time 0 sec. Drain time 1 sec. Ethane/propane mix. Grid: Quantifoil Cu 2/2 200. Add gold to cells prior to freezing: 1ul of gold to 4 ul of cells, freeze.T dga2017-09-25-100_slicer19025.jpg: multilayer structure beneath the inner membrane dga2017-09-25-100_slicer19026.jpg: multilayer structure beneath the inner membrane dga2017-09-25-100_slicer19027.jpg: multilayer structure beneath the inner membrane dga2017-09-25-100_slicer19028.jpg: last stage dividing cells? ; Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/dga2017-09-25-100 Cb_vero_201709250100.mrc, Tilt Series (Pixel Size 0.39 nm), 1.7 GB Download Cb_vero_201709250100_part61_6.rec, Reconstruction (Pixel Size 1.56 nm), 445.4 MB Download