Contributors: Institut de Recherche en Horticulture et Semences (IRHS); Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro); Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale) (GQE-Le Moulon); AgroParisTech-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Dynamique et Evolution des Parois cellulaires végétales; Laboratoire de Recherche en Sciences Végétales (LRSV); Université Toulouse III - Paul Sabatier (UT3); Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP); Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3); Université de Toulouse (UT); Génie Enzymatique et Cellulaire (GEC); Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS); Génie Enzymatique et Cellulaire. Reconnaissance Moléculaire et Catalyse - UMR CNRS 7025 (GEC UPJV); Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS); Graduate University of Advanced Technology, Kerman; GEC UTC (GEC UTC); Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS); Angers University and INRAE : the regional program “Objectif Végétal, Research, Education and Innovation in Pays de la Loire”, supported by the French region Pays de la Loire, Angers Loire Métropole and the European Regional Development Fund. Claudia Herrera-León’s PhD scholarship : the National Council for Science and Technology (CONACYT).; ANR-20-CE20-0025,STRESS-PEPT,Caractérisation de peptides sécrétés impliqués dans les réponses aux stress biotiques chez Arabidopsis(2020)
نبذة مختصرة : International audience ; The Arabidopsis PROSCOOP genes belong to a family predicted to encode secreted pro-peptides, which undergo maturation steps to produce peptides named SCOOP. Some of them are involved in defence signalling through their perception by a receptor complex including MIK2, BAK1 and BKK1. Here, we focused on the PROSCOOP10 gene, which is highly and constitutively expressed in aerial organs. The MS/MS analyses of leaf apoplastic fluids allowed the identification of two distinct peptides (named SCOOP10#1 and SCOOP10#2) covering two different regions of PROSCOOP10. They both possess the canonical S-X-S family motif and have hydroxylated prolines. This identification in apoplastic fluids confirms the biological reality of SCOOP peptides for the first time. NMR and molecular dynamics studies showed that the SCOOP10 peptides, although largely unstructured in solution, tend to assume a hairpin-like fold, exposing the two serine residues previously identified as essential for the peptide activity. Furthermore, PROSCOOP10 mutations led to an early-flowering phenotype and increased expression of the floral integrators SOC1 and LEAFY, consistent with the de-regulated transcription of PROSCOOP10 in several other mutants displaying early- or late-flowering phenotypes. These results suggest a role for PROSCOOP10 in flowering time, highlighting the functional diversity within the PROSCOOP family.
Processing Request
No Comments.