Contributors: University of Wisconsin-Madison; Trafic membranaire et Division cellulaire - Membrane Traffic and Cell Division; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité); Collège Doctoral; Sorbonne Université (SU); Harvard Medical School Boston (HMS); University of Utah; Boston University School of Medicine (BUSM); Boston University Boston (BU); A.R.S. is supported by the National Institutes of Health (R01 GM139695-01A1). Imaging was performed at the University of Wisconsin-Madison Biochemistry Optical Core, Wisconsin, United States, which was established with support from the University of Wisconsin-Madison Department of Biochemistry Endowment. J.S. is funded by an NIH Transformative R01 NS115716 and a Chan Zuckerberg Initiative Ben Barres Early Career Acceleration Award. M.B. was supported by the National Institutes of Health R01 GM122893 and GM144352. Part of this work has been supported by Institut Pasteur, CNRS, and ANR (Cytosign, SeptScort) to A.E., and A.P. received a fellowship from the Doctoral School Complexite´ du Vivant ED515, contrat n 2611 bis/2016 and Fondation ARC pour la recherche sur le cancer (DOC20190508876).; ANR-16-CE13-0004,CYTOSIGN,Polarité épithéliale et signalisation au cours de la cytodiérèse des cellules animales(2016); ANR-20-CE11-0014,SeptScort,Interaction spatiale et fonctionnelle entre septines et Escrt pendant la cytocinèse: une approche multi-échelle(2020)
نبذة مختصرة : International audience ; Long ignored as a vestigial remnant of cytokinesis, the mammalian midbody (MB) is released post-abscission inside large extracellular vesicles called MB remnants (MBRs). Recent evidence suggests that MBRs can modulate cell proliferation and cell fate decisions. Here, we demonstrate that the MB matrix is the site of ribonucleoprotein assembly and is enriched in mRNAs that encode proteins involved in cell fate, oncogenesis, and pluripotency, which we are calling the MB granule. Both MBs and post-abscission MBRs are sites of spatiotemporally regulated translation, which is initiated when nascent daughter cells re-enter G1 and continues after extracellular release. MKLP1 and ARC are necessary for the localization and translation of RNA in the MB dark zone, whereas ESCRT-III is necessary to maintain translation levels in the MB. Our work reveals a unique translation event that occurs during abscission and within a large extracellular vesicle.
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