Cytotoxic Effects of Methanol Extracts of Soursop Leaves (Annona muricata) on MCF-7 Cell Line and Its Effect on Expression of bcl-2

The leaves of the soursop (Annona muricata) contain annonaceus acetogenin that have cytotoxic effects on pancreatic carcinoma (PACA-2) and colon adenocarcinoma (HT- 29) cell lines. The purpose of this study was to determine the effects of cytotoxic of methanol leaf extract of soursop in MCF-7 breast cancer cell line. The methanol extract of soursop leaves was obtained by maceration using methanol for 3 x 24 hours. Cytotoxic activity was examined by MTT assay. The percent of living cells analyzed using the probit in SPSS (Statistical Product and Service Solution) program to obtain the IC50 values. The expression of Bcl-2 protein was observed by immunocytochemistry method. The results showed that the methanol extracts of soursop leaves has a cytotoxic activity in MCF-7 breast cancer cell line with IC50 values of 88,53 μg/mL. The methanol extract of soursop leaves decreasing Bcl-2 protein expression.