Histological Study of SlNPV Infection on Body Weight and Peritrophic Membrane Damage of Spodoptera Litura Larvae

Sanjaya, Machmudin D, Kurniawati ND. 2010. Histological study of SlNPV infection on body weight and peritrophicmembrane damage of Spodoptera litura larvae. Nusantara Bioscience 2: 135-140. The effect of SlNPV infection on body weight andperitrophic membrane damage of Spodoptera litura Fab. larvae has been carried out. The method was used Probit analysis, and based onLD 50 the virus was infected to know body weight and post infection damage.The damage of histological structure caused by SlNPV (0,315, 390, 465, 540 dan 615 PIB/mL) was investigated after 0, 12, 24, 72 and 96 hours post infection. The histological material wasprepared by using parafin method after fixation with Bouin Solution, then slice into 7 um and colored with Hematoxilin-Eosin. Theresult showed that the exposure SlNPV cause decreasing food consumption especially on 540 PIB/mL give average rate as amount of0.1675 mg. The descriptive obsevation on structural intact of peritrophic membrane histology caused by SlNPV infection shows atendency to decrease, while in control, there was no damage at all. The longer the exposition of virion in the midgut lumen the moredamage on peritrophic membrane occurred. The severest damage occurred 96 hour after infection. The result prove that haNPV virioncan destroy hystological structure of midgut.