State widths, dwell times, and distance between states of VSV inclusion body tracks in control cells and cells treated with latrunculin A.

(A) State widths for individual inclusion bodies in control and latrunculin A-treated cells in Fig 2C were calculated as the averages of the two axes in ellipses similar to those in Fig 3A and 3B , and are shown as histograms. States in latrunculin A-treated cells (n = 1118) were significantly larger than those in control cells (n = 1080) (p < 0.05, Mann-Whitney test). (B) Inclusion body dwell times within states were calculated as the time (number of frames) from one hop between states to the next in data similar to those in Fig 3C and 3D and are shown as histograms. There was no significant difference between dwell times of inclusion bodies in control cells (n = 1085 dwell times) versus latrunculin A-treated cells (n = 1093 dwell times) (p > 0.05, Mann-Whitney test). (C) The distances between states for individual inclusion bodies in control and latrunculin A-treated cells were calculated as the distance from the center of one state to the center of the next and are shown as histograms. State-to-state distances of inclusion bodies in latrunculin A-treated cells (n = 927 distances) were significantly larger than those in control cells (n = 893 distances) (p < 0.05, Mann-Whitney test).