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Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro : Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry

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  • معلومة اضافية
    • بيانات النشر:
      Malmö universitet, Institutionen för biomedicinsk vetenskap (BMV)
      Malmö universitet, Biofilms Research Center for Biointerfaces
      Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic
      College of Chemistry and Chemical Engineering, Yan’an University, Yan’an 215123, China
      Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden
      Basel
    • الموضوع:
      2022
    • Collection:
      Malmö University Electronic Publishing (MUEP)
    • نبذة مختصرة :
      Colorectal cancer (CRC) is the second most metastatic disease with the majority of cases detected in Western countries. Metastases are formed by circulating altered phenotype tumor cells causing 20% of CRC related deaths. Metastatic cells may show higher expression of surface molecules such as CD44, and changes in morphological properties are associated with increased invasiveness and poor prognosis. In this study, we intended to mimic the environment for metastasizing cells. Here, we used digital holographic cytometry (DHC) analysis to determine cellular morphological properties of three metastatic and two non-metastatic colorectal cancer cell lines to show differences in morphology between the CRC cells and peripheral blood mononuclear cells (PBMCs). By establishing differences in cell area, cell thickness, cell volume, and cell irregularity even when the CRC cells were in minority (5% out of PBMCs), DHC does discriminate between CRC cells and the PBMCs in vitro. We also analyzed the epithelial marker EpCAM and migration marker CD44 using flow cytometry and demonstrate that the CRC cell lines and PBMC cells differ in EpCAM and CD44 expression. Here, we present DHC as a new powerful tool in discriminating cells of different sizes in suspension together with a combination of biomarkers.
    • File Description:
      application/pdf
    • Relation:
      Photonics, 2304-6732, 2022, 9:12; orcid:0000-0002-5882-4155; orcid:0000-0001-7682-7678; orcid:0000-0001-5463-3338; orcid:0000-0002-0841-5804; orcid:0000-0002-2993-0354; http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-58269; ISI:000902845600001; Scopus 2-s2.0-85144643845
    • الرقم المعرف:
      10.3390/photonics9120955
    • Rights:
      info:eu-repo/semantics/openAccess
    • الرقم المعرف:
      edsbas.BA62FE88