EFFECT OF 50% ETHANOLIC EXTRACT OF PEGAGAN HERB (Centella asiatica (L.) Urban) ON CELL PROLIFERATION OF LYMPHOCYTES IN Balb/c MALE MICE INDUCED BY HEPATITIS B VACCINE ; PENGARUH EKSTRAK ETANOLIK 50% HERBA PEGAGAN (Centella asiatica (L.) Urban) TERHADAP PENINGKATAN PROLIFERASI SEL LIMFOSIT MENCIT JANTAN GALUR Balb/c YANG DIINDUKSI VAKSIN HEPATITIS B

Pegagan (Centella asiatica (L.) Urban) has been known as multi effect plant that empirically used in the traditional medicine. This research aims to evaluate the effect of 50%ethanolic extract of pegagan herb against increased lymphocyte proliferation cells in Balb/c mice that induced with hepatitis B vaccine, and also to evaluate triterpen glicosida compound (asiaticosida) of 50% ethanolic extract of pegagan herb. The test was done by 60 Balb/c male mices that divided into 6 groups : control group, testing group that was treated with pegagan extract dose of 25 mg/KgBB, 50 mg/KgBB, 100 mg/KgBB, 200 mg/KgBB and 400 mg/KgBB. All of mices were vaccinated on day 0 and days 15. Furthermore, identification of triterpen glicosida compound was done by KLT method using BAW 4:1:5 as upper eluen. Lymphocyte cells were isolated and the cells proliferation measured by MTT reduction method. Data was analyzed statistically using 95% confidence level. The result shown that 50% of ethanolic extract of pegagan herb did not give the immuomodulatory effect while the identification test shown that 50% of extract of pegagan herb has triterpen glicosida compound (asiaticoside). ; Pegagan (Centella asiatica (L.) Urban) dikenal sebagai tanaman yang memiliki banyak khasiat yang secara empiris digunakan dalam pengobatan tradisional. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian ekstrak etanolik 50% herba pegagan terhadap peningkatan proliferasi sel limfosit pada mencit jalur Balb/c yang diinduksi vaksin hepatitis B serta untuk mengetahui adanya senyawa glikosida triterpen (asiatikosida) dalam ekstrak etanolik 50% herba pegagan. Sebanyak 60 ekor mencit jantan galur Balb/c dibagi menjadi 6 kelompok yaitu kelompok kontrol, kelompok uji yang diberi ekstrak pegagan dosis 25 mg/KgBB, 50 mg/KgBB, 100 mg/KgBB, 200 mg/KgBB dan 400 mg/KgBB. Hewan uji divaksinasi pada hari ke-0 dan hari ke-15. Sementara identifikasi senyawa glikosida triterpen dilakukan dengan metode KLT dengan fase gerak atas BAW 4:1:5. Sel limfosit diisolasi ...