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The effects of hyaluronate-containing medium on human embryo attachment to endometrial epithelial cells in vitro

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  • معلومة اضافية
    • الموضوع:
      2020
    • Collection:
      The University of Manchester: Research Explorer - Publications
    • نبذة مختصرة :
      STUDY QUESTION Does embryo transfer medium containing hyaluronate (HA) promote the attachment phase of human embryo implantation? SUMMARY ANSWER HA-containing medium does not promote human blastocyst attachment to endometrial epithelial cells in vitro. WHAT IS KNOWN ALREADY Embryo transfer media containing high concentrations of HA are being used to increase implantation and live birth rates in IVF treatment, although the mechanism of action is unknown. STUDY DESIGN, SIZE, DURATION Expression of HA-interacting genes in frozen-thawed oocytes/embryos was assessed by microarray analysis (n = 21). Fresh and frozen human blastocysts (n = 98) were co-cultured with human endometrial epithelial Ishikawa cell layers. Blastocyst attachment and the effects of a widely used HA-containing medium were measured. PARTICIPANTS/MATERIALS, SETTING, METHODS Human embryos surplus to treatment requirements were donated with informed consent from several ART centres. Blastocyst-stage embryos were transferred at day 6 to confluent Ishikawa cell layers; some blastocysts were artificially hatched. Blastocyst attachment was monitored from 1 to 48 h, and the effects of blastocyst pre-treatment for 10 min with HA-containing medium were determined. MAIN RESULTS AND THE ROLE OF CHANCE Human embryos expressed the HA receptor genes CD44 and HMMR, hyaluronan synthase genes HAS1–3, and hyaluronidase genes HYAL1–3, at all stages of preimplantation development. Attachment of partially hatched blastocysts to Ishikawa cells at 24 and 48 h was related to trophectoderm grade (P = 0.0004 and 0.007, respectively, n = 34). Blastocysts of varying clinical grades that had been artificially hatched were all attached within 48 h (n = 21). Treatment of artificially hatched blastocysts with HA-containing medium did not significantly affect attachment at early (1–6 h) or late (24 and 48 h) time points, compared with control blastocysts (n = 43). LIMITATIONS, REASONS FOR CAUTION Using an adenocarcinoma-derived cell line to model embryo-endometrium attachment may ...
    • Relation:
      https://research.manchester.ac.uk/en/publications/6a97348b-122e-49c8-bf4c-8793d3782843
    • الرقم المعرف:
      10.1093/hropen/hoz033
    • الرقم المعرف:
      10.1093/hropen/hoz033/5762317
    • الدخول الالكتروني :
      https://research.manchester.ac.uk/en/publications/6a97348b-122e-49c8-bf4c-8793d3782843
      https://doi.org/10.1093/hropen/hoz033
      https://academic.oup.com/hropen/article/doi/10.1093/hropen/hoz033/5762317
    • Rights:
      info:eu-repo/semantics/openAccess
    • الرقم المعرف:
      edsbas.B8B0DBEC