Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

How to increase freeze-substitution and electron microscopy embedding reliability

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • معلومة اضافية
    • بيانات النشر:
      Walter de Gruyter GmbH
    • الموضوع:
      2024
    • نبذة مختصرة :
      Freeze substitution is a crucial technique in electron microscopy, enabling the preservation of biological specimen ultrastructure. However, existing tools are primarily designed for 3 mm carriers, limiting their compatibility with larger carriers such as CryoCapsules or 6 mm carriers. In this study, we developed a novel freeze substitution tool, simply named the FS-basket, to address the challenges associated with embedding samples vitrified in larger carriers. The tool comprises a mesh with pore sizes smaller than the sample chunks, deposited below the carrier to maintain a tight seal while allowing for easy fluid exchange.
    • الرقم المعرف:
      10.1515/mim-2024-0004
    • الرقم المعرف:
      10.1515/mim-2024-0004/xml
    • الرقم المعرف:
      10.1515/mim-2024-0004/pdf
    • الدخول الالكتروني :
      http://dx.doi.org/10.1515/mim-2024-0004
      https://www.degruyter.com/document/doi/10.1515/mim-2024-0004/xml
      https://www.degruyter.com/document/doi/10.1515/mim-2024-0004/pdf
    • Rights:
      http://creativecommons.org/licenses/by/4.0
    • الرقم المعرف:
      edsbas.B7D6E2FC