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Additional file 1 of Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L.

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  • معلومة اضافية
    • الموضوع:
      2022
    • Collection:
      Smithsonian Institution: Digital Repository
    • نبذة مختصرة :
      Additional file 1: Figure S1. In vitro culture of M. azedarach L.; A1: leaf explants, B1: viable and browning leaf explants after disinfection and in vitro culture, C1: swollen leaf explants and initiation of callus induction on MS medium containing 1 mg/L NAA + 1 mg/L Kin, D1 and E1: callus induced on MS medium containing 1 mg/L NAA + 1 mg/L BAP and 3 mg/L NAA + 1 mg/L BAP (is embryogenic callus), respectively, A2 and B2: callus initiation and growth on MS + 1 mg/L 2,4-D + 1 mg/L Kin, C2, D2 and E2: callus growth on MS medium containing 1 mg/L 2,4-D + 1 mg/L Kin, 1 mg/L NAA + 1 mg/L Kin and 3 mg/L 2,4-D + 3 mg/L BAP, respectively. Figure S2. In vitro culture of M. azedarach L.; A1: callus induction on MS medium containing 3 mg/L NAA + 1 mg/L Kin containing green cells in callus and B1: 3 mg/L NAA + 1 mg/L BAP containing green and globular shape spots in callus (are embryogenic callus), C1: embryogenic callus that have taken root, A2: globular and torpedo shape embryos, B2: globular embryos under a stereoscope and C2: torpedo shape embryos under a stereoscope. Figure S3. In vitro culture of M. azedarach L.; Callus growth on MS + A1: 1 mg/L NAA + 1 mg/L Kin, B1: 1 mg/L NAA + 1 mg/L BAP, C1: 1 mg/L 2,4-D + 1 mg/L Kin, D1: 1 mg/L 2,4-D + 1 mg/L BAP, A2: 3 mg/L NAA + 1 mg/L Kin, B2: 3 mg/L NAA + 1 mg/L BAP, C2: 3 mg/L NAA + 3 mg/L Kin, D2: 3 mg/L NAA + 3 mg/L BAP, A3: 3 mg/L 2,4-D + 1 mg/L Kin, B3: 3 mg/L 2,4-D + 1 mg/L BAP, C3: 3 mg/L 2,4-D + 3 mg/L Kin, D3: 3 mg/L 2,4-D + 3 mg/L BAP, A4: 5 mg/L NAA + 1 mg/L Kin, B4: 5 mg/L NAA + 1 mg/L BAP, C4: 5 mg/L NAA + 5 mg/L Kin, D4: 5 mg/L NAA + 5 mg/L BAP, A5: 5 mg/L 2,4-D + 1 mg/L Kin, B5: 5 mg/L 2,4-D + 1 mg/L BAP, C5: 5 mg/L 2,4-D + 5 mg/L Kin, D5: 5 mg/L 2,4-D + 5 mg/L BAP, respectively. Figure S4. HPLC chromatogram of rutin, quercetin and kaempferol in the standard mixture (A) and M. azedarach L. calli grown on the MS medium containing 3 mg/L NAA + 3 mg/L Kin (B). Table S1. Different disinfection methods used in experiment 1 for sterilization of M. azedarach L. leaf ...
    • Relation:
      https://figshare.com/articles/journal_contribution/Additional_file_1_of_Sterilization_protocols_and_the_effect_of_plant_growth_regulators_on_callus_induction_and_secondary_metabolites_production_in_in_vitro_cultures_Melia_azedarach_L_/18132751
    • الرقم المعرف:
      10.6084/m9.figshare.18132751.v1
    • Rights:
      CC BY 4.0
    • الرقم المعرف:
      edsbas.B6357F89