Protocatechuate 3,4-dioxygenase from thermophilic Geobacillus sp. strain ; Geobacillus sp. Protokatechuato 3,4-dioksigenazė

Protocatechuate 3,4-dioxygenase ( EC 1.13.11.3 ) catalyses the ring cleavage step in catabolism of aromatic compounds through the protocatechuate branch of the b-ketoadipate pathway. The protocatechuate 3,4-dioxygenase was purified to homogeneity from the thermophilic Geobacillus strain grown on naphthalene for the first time. The enzyme was purified about 24-fold with a specific activity of 34.2 U mg of protein-1 by a purification procedure including ammonium sulfate fractionation and column chromatographies on DEAE– cellulose and hydroxylapatite. The relative molecular mass of the native enzyme estimated on gel chromatography of Sephadex G-200 was 480 kDa. The pH and temperature optima for enzyme activity were 8 and 60 oC, respectively. A half-live of the protocatechuate 3,4-dioxygenase at the optimum temperature was 40 min. The kinetic parameters of the Geobacillus strain protocatehuate 3,4-dioxygenase were determinated. The enzyme gave typical saturation kinetics and had an apparent Km of 7 mM for protocatechuate and 33 mM for catechol.