Selected issues of non-conventional enzymology Part I - Characterisation of enzymatic processes carried out in amedium with subcritically low water content ; Wybrane zagadnienia z enzymologiiniekonwencjonalnej Część I - Charakterystyka procesów enzymatycznych prowadzonych w środowisku o subkrytycznie niewielkiej zawartości wody

Selected issues of non-conventional enzymology ; Enzymology of non-aqueous media, also termed a non-conventionalenzymology, is a discipline focused on chemical reactions catalysed by enzymesin the media other than water. However, such environments always contain certain amount ofwater, either dissolved in a solvent or enzyme-bound. The latterfraction of water is called an essential one, and it is crucial for the non-aqueousenzymology, since it stabilises the conformation of protein molecule and determines its enzymatic activity. The paper presents structure, function, and methods of assays of the enzyme-bound water together with other water forms thatcan also be found in reaction milieu. The methods of regulation of enzymaticactivity in non-aqueous media by using compounds capable of associating withpolar atoms of amino acid residues involved in catalysis, and thus positively influencing activity of enzymes, have also been discussed. The factors giving riseto a decrease in catalytic activity of enzymes under non-aqueous conditions,and methods of prevention against this phenomenon have also been analysed. ; Wybrane zagadnienia z enzymologii niekonwencjonalnej ; Enzymology of non-aqueous media, also termed a non-conventionalenzymology, is a discipline focused on chemical reactions catalysed by enzymesin the media other than water. However, such environments always contain certain amount ofwater, either dissolved in a solvent or enzyme-bound. The latterfraction of water is called an essential one, and it is crucial for the non-aqueousenzymology, since it stabilises the conformation of protein molecule and determines its enzymatic activity. The paper presents structure, function, and methods of assays of the enzyme-bound water together with other water forms thatcan also be found in reaction milieu. The methods of regulation of enzymaticactivity in non-aqueous media by using compounds capable of associating withpolar atoms of amino acid residues involved in catalysis, and thus positively influencing activity of ...