Detection of H7N7-HP HAtag and H7N7-LP FLAGtag in tissues of chickens and ducks at 3 dpi.

(A, B) Viral RNA quantification in tissues harvested at 3 dpi from chickens (A) and ducks (B) inoculated with ~10 4 EID 50 of H7N7-HP HAtag or ~10 6 EID 50 of H7N7-LP FLAGtag . Viral RNA amounts were determined by RT-qPCR targeting the influenza virus matrix segment and expressed as 40-cycle threshold (Ct) normalized/gram tissue. Bars represent the arithmetic mean. The dotted lines distinguish tissues from the respiratory, digestive, and miscellaneous systems. (C, D) Infectious virus titers in tissues from panels (A, B). Infectious titers were determined by endpoint titration in MDCK cells and expressed as log 10 TCID 50 /gram tissue. Bars represent the arithmetic mean of log 10 transformed values. Dotted lines similar to (A, B). The horizontal solid lines indicate the limit of detection of the endpoint titration assay per tissue.