The effect of Curcuma longa on fasting blood glucose, MMP-9 and IFN-γ in diabetes mellitus: an experimental study

Background: Turmeric (Curcuma longa) contains curcumin as an active ingredient that has ability as a potent antioxidant and antiinflammation. Turmeric extract can improve glucose level and surpress micro and microangiopathy complication in Diabetes Mellitus Type 2. The aim of this study was to investigate the role of turmeric extract administration on fasting glucose level, MMP-9, and IFN-γ. Methods: Experimental PostTest Only Control Group Design study was conducted. Twenty four Wistar rats aged 2 months weighing 180-200 grams were adapted for 2 days, divided into 4 groups randomly: negative control group induced by STZ-NA only (P1), positive control group induced by STZ-NA and received metformin 45mg/kgBW/day (P2), treatment group induced by STZ-NA and received turmeric extract 200mg/kgBW/day (K1), and ), treatment group induced by STZ-NA and received combination of metformin 22,5mg/kgBW/day and turmeric extract 100mg/kgBW/day. The levels of fasting glucose serum were measured by spectrophotometric method, while ELISA measured the levels of MMP-9 and IFN-γ. Results: Mean + SD Fasting blood glucose level was decreased respectively from K1, K2, P1 and P2 (277.00 + 4.00, 100.00 + 2.00, 98.04 + 3.00, 92.00 + 3.00 mg/dl). MMP-9 level was decreased respectively from K1, K2, P1 and P2 (653.09 + 18.00, 328.00 + 14.00, 156.00 + 4.00, 131.00 + 4.00 ng/ml). IFN–γ level was decreased respectively from K1, K2, P1 and P2 (300 + 16.00, 85.00 + 3.00, 61.05 + 2.04, 49.00 + 1.09 ng/ml.) Kruskal Wallis test for Fasting blood glucose, MMP-9, dan IFN–γ shows significant differences among groups. (p=0.004, p=0.004, dan p=0.004). Using post hoc test, all groups significantly differ with p=0.000. Conclusion: The administration of turmeric extract improves fasting glucose level, MMP-9, and IFN-γ in type 2 DM Wistar rats