CHARACTERISTICS OF THE DISTRIBUTION OF ALLELIC POLYMORPHISM OF PRO-INFLAMMATORY CYTOKINE GENES (TNF-α (G308A), IL2 (T330G), IL6 (C174G), IL10 (C592A)) IN PURULENT-INFLAMMATORY DISEASES OF THE MIDDLE EA

Objective: Study of the distribution of single nucleotide polymorphisms of the genes of pro- inflammatory cytokines involved in the initiation of inflammation - TNF-α (G308A), IL2 (T330G), IL6 (C174G) and IL10 (C592A) among patients with pyoinflammatory diseases of the middle ear Methods: The material for the study of pus released from the middle ear was performed in all patients with a diagnosis of CHSO who were admitted to the hospital for treatment. All genotypic variants of the studied genetic polymorphisms (TNF-α (G308A), IL2 (T330G), IL6 (C174G), IL10 (C592A) in the studied groups were analyzed by Hardy-Weinberg equilibrium (RHB, p>0.05). DNA extraction was carried out using the AmpliPrim RIBO-prep commercial kit (JSC Interlabservis, Russia). The OpenEpi 2009, version 2.3 software package was used as a tool for calculating the results of DNA extraction. DNA extraction was carried out using the AmpliPrim RIBO-prep commercial kit (JSC Interlabservis, Russia). According to the instructions, 30 mkl of the required sorbent was added to a special laboratory container (test tube) with 1–2 ml of biomaterial. The resulting mixture was stirred in a vortex mixer and left at a temperature of 600°C for 5 minutes. Molecular markers were detected on a Rotor Gene Q device (Quagen, Germany) by allele-specific PCR in Real-Time format using the NPF Litex assembly (Russia). Results: In the general group of patients (Group I), the observed frequencies of the G/G and G/A genotype variants were 0.87 and 0.13, respectively, depending on the distribution of the proportion of the TNF-α single nucleotide polymorph. (G308A) genotypes. Their unexpected frequencies were0.88 and 0.12, respectively. At the same time, the frequencies of the A/A mutant genotype are zero. At the same time, it should be noted that the difference between the frequencies of Ho and He of the G/G and G/A genotypes is not statistically significant (χ2=0.4; P=0.506; df=1), which means that in the canonical distribution of RXB (p>0 .05) showed no difference. ...