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An Estradiol-Inducible Promoter Enables Fast, Graduated Control of Gene Expression in Fission Yeast [preprint]

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Contributors:
      Department of Biochemistry and Molecular Pharmacology
    • الموضوع:
      2022
    • Collection:
      University of Massachusetts, Medical School: eScholarship@UMMS
    • نبذة مختصرة :
      The fission yeast Schizosaccharomyces pombe lacks a diverse toolkit of inducible promoters for experimental manipulation. Available inducible promoters suffer from slow induction kinetics, limited control of expression levels and/or a requirement for defined growth medium. In particular, no S. pombe inducible promoter systems exhibit a linear dose response, which would allow expression to be tuned to specific levels. We have adapted a fast, orthogonal promoter system with a large dynamic range and a linear dose response, based on β-estradiol-regulated function of the human estrogen receptor, for use in S. pombe. We show that this promoter system, termed Z3EV, turns on quickly, can reach a maximal induction of 20 fold, and exhibits a linear dose response over its entire induction range, with few off target effects. We demonstrate the utility of this system by regulating the mitotic inhibitor Wee1 to create a strain in which cell size is regulated by β-estradiol concentration. This promoter system will be of great utility for experimentally regulating gene expression in fission yeast.
    • Relation:
      Now published in Yeast doi: 10.1002/yea.3235 .; bioRxiv 092338; doi: https://doi.org/10.1101/092338. Link to preprint on bioRxiv service.; http://hdl.handle.net/20.500.14038/29328; https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2564&context=faculty_pubs&unstamped=1; https://escholarship.umassmed.edu/faculty_pubs/1554; faculty_pubs/1554
    • الرقم المعرف:
      10.1101/092338
    • الدخول الالكتروني :
      https://doi.org/10.1101/092338
      https://hdl.handle.net/20.500.14038/29328
      https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=2564&context=faculty_pubs&unstamped=1
      https://escholarship.umassmed.edu/faculty_pubs/1554
    • Rights:
      The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license. ; http://creativecommons.org/licenses/by-nc-nd/4.0/
    • الرقم المعرف:
      edsbas.AFA68E7C