The effects of pressure on the buoyant behavior of deoxyribonucleic acid and tobacco mosaic virus in a density gradient at equilibrium in the ultracentrifuge

In the original analysis(1) of the behavior of macromolecules and viruses in a buoyant density gradient at equilibrium in the ultracentrifuge, all components were assumed to be incompressible. As a few hundred atmospheres are normally generated in the liquid, it is to be expected that previous results based on the assumption of incompressibility require re-examination. ; © 1961 by the National Academy of Sciences. Communicated by Linus Pauling, May 29, 1961. This investigation was supported in part by Research Grant H-3394 from the National Institutes of Health, U.S. Public Health Service. [J.E.H. was a] Predoctoral Fellow of the National Science Foundation. [J.B.I. was a] U.S. Public Health Service Research Fellow of the Division of General Medical Sciences. Gates and Crellin Laboratories of Chemistry, Contribtion No. 2701.