Elevated non-esterified fatty acid concentrations during in vitro maturation affect the transcriptome profile of day 14 bovine embryos 7 days after transfer

A235E Embryology, developmental biology, and physiology of reproduction ; National audience ; We showed earlier that exposure to elevated non-esterified fatty acid (NEFA) concentrations during in vitro oocyte maturation (IVM) affects post-hatching development of day (D) 14 bovine embryos (Desmet et al., Anim. Reprod. 14,p947,2017). Lipotoxic conditions during IVM influence DNA methylation in the D7 embryo and may thus affect its transcriptome during later development. Therefore the aim was to analyse the transcriptome profile of D14 embryos to investigate which mechanisms mediate carryover effects of adverse maturation condition on post-hatching development. Bovine oocytes were matured for 24h under 2 conditions: 1) basal physiological NEFA conditions (BAS; 28μM stearic acid (SA), 21μM oleic acid (OA), 23μM palmitic acid (PA)); and 2) high PA concentration (most predominant in follicular fluid during negative energy balance) (HPA; 150μM PA, 28µM SA, 21µM OA). After fertilization, zygotes were cultured in SOF with serum. 8 blastocysts (normal and expanded, equally distributed per treatment and per replicate) were transferred to healthy non-lactating Holstein Friesian cows at D7 (n = 8, 5 replicates). Each cow was used once for each treatment in a cross-over design. After transcervical recovery, D14 concepti (n = 45) were dissected into embryonic disc (ED) and extra-embryonic tissue (EXT). ED (n = 11BAS/7PA) and EXT (n = 13BAS/8PA) were subjected to RNA sequencing (without RNA amplification). Differential expression was established in a DESeq2 model based on Negative Binomial distribution. Samples were divided by sample type for further analysis. A false discovery rate (FDR) of 10% was used as cut-off for differentially expressed genes (DEG) and P-values were Benjamini-Hochberg corrected. Ingenuity Pathway Analysis (IPA) and Gene Set Enrichment Analysis (GSEA) were performed. Recovery rate at D14 was not significantly different between treatments. Within ED and EXT datasets, only 14 and 0 DEGs were detected in HPA ...