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Development of molecular and cellular tools to decipher the type I IFN pathway of the common vampire bat

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Contributors:
      Laboratoire des Interactions Virus-Hôtes Cayenne, Guyane Française; Institut Pasteur de la Guyane; Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP); Hépacivirus et immunité innée; Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS); This study was conducted within the CAROLIA and BATIMMUNE programs. CAROLIA was supported by European funds (ERDF/FEDER) and assistance from the Region Guyane and the Direction Regionale pour la Recherche et la Technologie. BATIMMUNE wasfunded by the Institut Pasteur through a Transversal Research Program (PTR499). This study also received a European Commission "REGPOT-CT-2011-285837-STRonGer" grant within the FP7 and an "Investissement d'Avenir " grant managed by the Agence Nationale de la Recherche (CEBA, Ref. ANR-10-LABEX-25-01).; ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010); European Project: 285837,EC:FP7:REGPOT,FP7-REGPOT-2011-1,STRONGER(2011)
    • بيانات النشر:
      HAL CCSD
      Elsevier
    • الموضوع:
      2017
    • Collection:
      Institut Pasteur: HAL
    • نبذة مختصرة :
      International audience ; Though the common vampire bat, Desmodus rotundus, is known as the main rabies virus reservoir in Latin America, no tools are available to investigate its antiviral innate immune system. To characterize the IFN-I pathway, we established an immortalized cell line from a D. rotundus fetal lung named FLuDero. Then we molecularly characterized some of the Toll-like receptors (TLR3, 7, 8 and 9), the three RIG-I-like receptor members, as well as IFNa1 and IFNb. Challenging the FLuDero cell line with poly (I:C) resulted in an up-regulation of both IFNa1 and IFNb and the induction of expression of the different pattern recognition receptors characterized. These findings provide evidence of the intact dsRNA recognition machinery and the IFN-I signaling pathway in our cellular model. Herein, we generated a sum of insightful specific molecular and cellular tools that will serve as a useful model to study virusehost interactions of the common vampire bat.
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/29122634; info:eu-repo/grantAgreement/EC/FP7/285837/EU/Strengthening transdisciplinary research on infectious and emerging diseases in French Guiana: linking fieldwork, benchside and bedside/STRONGER; pasteur-01633796; https://riip.hal.science/pasteur-01633796; https://riip.hal.science/pasteur-01633796/document; https://riip.hal.science/pasteur-01633796/file/2018%20Sarkis%20et%20al%20DCI.pdf; PUBMED: 29122634
    • الرقم المعرف:
      10.1016/j.dci.2017.10.023
    • الدخول الالكتروني :
      https://riip.hal.science/pasteur-01633796
      https://riip.hal.science/pasteur-01633796/document
      https://riip.hal.science/pasteur-01633796/file/2018%20Sarkis%20et%20al%20DCI.pdf
      https://doi.org/10.1016/j.dci.2017.10.023
    • Rights:
      http://creativecommons.org/licenses/by-nc-nd/ ; info:eu-repo/semantics/OpenAccess
    • الرقم المعرف:
      edsbas.ADE0B337