نبذة مختصرة : It is highly desirable to develop a rapid and simple method to detect pathogens. Combining nanomaterials with electrochemical techniques is an efficient way for pathogen detection. Herein, a novel 3D Ag nanoflower was prepared via a biomineralization method by using bovine serum albumin (BSA) as a template. It was adopted as a sensing interface to construct an electrochemical bacteria immunosensor for the rapid detection of foodborne pathogens Escherichia coli ( E. coli ) O157:H7. Bacterial antibody was immobilized onto the surface of Ag nanoflowers through covalent conjugation. Electrochemical impedance spectroscopy (EIS) was used to detect and validate the resistance changes, where [Fe(CN) 6 ] 3−/4− acted as the redox probe. A linear relation between R et and E. coli concentration was obtained in the E. coli concentration range of 3.0 × 10 2 –3.0 × 10 8 cfu mL −1 . The as-prepared biosensor gave rise to an obvious response to E. coli but had no distinct response to Cronobacter sakazakii , methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus albus , Lactobacillus easei , and Shigella flexneri , revealing a high selectivity for the detection of the pathogens down to 100 cfu mL −1 in a short time. We believe that this BSA-conjugated 3D Ag nanoflowers could be used as a powerful interface material with good conductivity and biocompatibility for improving pathogen detection and treatment in the field of medicine, environment, and food safety.
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