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Combined effects of high fat diet and exercise on autophagy in white adipose tissue of mice

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  • معلومة اضافية
    • Contributors:
      Islamic Azad University of Hamedan; Tehran University of Medical Sciences (TUMS); University of Guilan; Alzahra University; University of British Columbia (UBC); University of Kurdistan Sanandaj - Iran (UOK); University of Freiburg Freiburg; Laboratoire Mouvement Sport Santé (M2S); Université de Rennes (UR)-École normale supérieure - Rennes (ENS Rennes)-Université de Brest (UBO)-Université de Rennes 2 (UR2)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ); Institut International des Sciences du Sport (2I2S); This work was supported by Tehran University of Medical Sciences and Health Services (no. 47823) and University of Ayatollah Alozma Boroujerdi (no. 15664-214256).
    • بيانات النشر:
      HAL CCSD
      Elsevier
    • الموضوع:
      2023
    • Collection:
      Archive Ouverte de l'Université Rennes (HAL)
    • نبذة مختصرة :
      International audience ; Aim: The effects of nutrition and exercise on autophagy are not well studied. This study aimed to investigate the combined effects of high-fat diets (HFD) and exercise training (ET) on autophagy in white adipose tissue of mice.Materials and methods: Male C57BL/6 mice were assigned into four groups of 7 mice per group: (1) Control, (2) high-fat diet-induced obesity (HFD-Ob), (3) exercise training (ET), and (4) high-fat diet with exercise training (HFD-ET). The HFD-Ob group was fed a high-fat diet for 14 weeks, while the ET group continuously ran on a treadmill for five sessions per week for seven weeks, and the HFD-ET group had both HFD and exercise training. qReal-time-PCR and western blot were used to measure the mRNA and protein levels of autophagy markers in white adipose tissue.Results: Mice from the HFD group showed higher levels in autophagy-related gene5 (ATG5, p = 0.04), ATG7 (p = 0.002), cathepsin B (CTSB, p = 0.0004), LC3-II (p = 0.03) than control. Mice in the ET group displayed higher levels of genes for ATG7 (p = 0.0003), microtubule-associated protein1-light chain 3 (LC3, p = 0.05), lysosome-associated membrane protein 2 (LAMP2, p = 0.04) and cathepsin L (CTSL, p = 0.03) than control. Mice from the HFD-ET group had higher levels of genes for ATG7 (p = 0.05) and CTSL (p = 0.043) and lower levels of genes for CTSB (p = 0.045) compared to the HFD group and lower levels of LAMP2 (p = 0.02) compared to the ET group.Conclusion: There were increases in autophagosome formation in the white adipose tissue from mice in the HFD and ET groups. A combination of HFD and ET enhances autophagosome formation and modulates lysosomal degradation in white adipose tissue.
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/36587790; hal-03930680; https://univ-rennes.hal.science/hal-03930680; https://univ-rennes.hal.science/hal-03930680/document; https://univ-rennes.hal.science/hal-03930680/file/Daneshyar%20et%20al.%20-%202022%20-%20Combined%20effects%20of%20high%20fat%20diet%20and%20exercise%20on%20.pdf; PUBMED: 36587790
    • الرقم المعرف:
      10.1016/j.lfs.2022.121335
    • Rights:
      http://creativecommons.org/licenses/by-nc/
    • الرقم المعرف:
      edsbas.A9E27AEC