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The olfactory secretome varies according to season in female sheep and goat

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  • معلومة اضافية
    • Contributors:
      Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF); Université de Lille-Centre National de la Recherche Scientifique (CNRS); Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL); Institut Pasteur de Lille; Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS); Physiologie de la reproduction et des comportements Nouzilly (PRC); Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation Saumur (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS); ANR PHEROMALE; Region Centre-Val de Loire; ANR-15-CE21-0012,PHEROMALE,L'effet mâle chez les ovins: Identification, mécanismes d'action et utilisation de phéromone(s) potentielle(s).(2015)
    • بيانات النشر:
      CCSD
      BioMed Central
    • الموضوع:
      2019
    • Collection:
      LillOA (HAL Lille Open Archive, Université de Lille)
    • نبذة مختصرة :
      International audience ; Background: Small ungulates (sheep and goat) display a seasonal breeding, characterised by two successive periods, sexual activity (SA) and sexual rest (SR). Odours emitted by a sexually active male can reactivate the ovulatory cycle of anoestrus females. The plasticity of the olfactory system under these hormonal changes has never been explored at the peripheral level of odours reception. As it was shown in pig that the olfactory secretome (proteins secreted in the nasal mucus) could be modified under hormonal control, we monitored its composition in females of both species through several reproductive seasons, thanks to a non-invasive sampling of olfactory mucus. For this purpose, two-dimensional gel electrophoresis (2D-E), western-blot with specific antibodies, MALDI-TOF and high-resolution (nano-LC-MS/MS) mass spectrometry, RACE-PCR and molecular modelling were used. Results: In both species the olfactory secretome is composed of isoforms of OBP-like proteins, generated by post-translational modifications, as phosphorylation, N-glycosylation and O-GlcNAcylation. Important changes were observed in the olfactory secretome between the sexual rest and the sexual activity periods, characterised in ewe by the specific expression of SAL-like proteins and the emergence of OBPs O-GlcNAcylation. In goat, the differences between SA and SR did not come from new proteins expression, but from different post-translational modifications, the main difference between the SA and SR secretome being the number of isoforms of each protein. Proteomics data are available via ProteomeXchange with identifier PXD014833.Conclusion: Despite common behaviour, seasonal breeding, and genetic resources, the two species seem to adapttheir olfactory equipment in SA by different modalities: the variation of olfactory secretome in ewe couldcorrespond to a specialization to detect male odours only in SA, whereas in goat the stability of the olfactorysecretome could indicate a constant capacity of odours detection ...
    • ISBN:
      978-0-00-500573-6
      0-00-500573-6
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/31666027; PRODINRA: 488936; PUBMED: 31666027; WOS: 000500573600003
    • الرقم المعرف:
      10.1186/s12864-019-6194-z
    • الدخول الالكتروني :
      https://hal.science/hal-02347395
      https://hal.science/hal-02347395v1/document
      https://hal.science/hal-02347395v1/file/2019%20BMC%20Genomics.pdf
      https://doi.org/10.1186/s12864-019-6194-z
    • Rights:
      http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess
    • الرقم المعرف:
      edsbas.A6E2FC7