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Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

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  • معلومة اضافية
    • Contributors:
      Ludwig Maximilian University Munich = Ludwig Maximilians Universität München (LMU); Heinrich Heine Universität Düsseldorf = Heinrich Heine University Düsseldorf; University of Sheffield Sheffield; B CUBE - Center for Molecular Bioengineering TU Dresden, Germany; Center for Molecular and Cellular Bioengineering TU Dresden, Germany (CMCB); Technische Universität Dresden = Dresden University of Technology (TU Dresden)-Technische Universität Dresden = Dresden University of Technology (TU Dresden); University of California (UC); University of Groningen Groningen; University of Regensburg; Howard Hughes Medical Institute (HHMI); Hasselt University (UHasselt); Catholic University of Leuven = Katholieke Universiteit Leuven (KU Leuven); Universität zu Lübeck = University of Lübeck Lübeck; Friedrich-Schiller-Universität = Friedrich Schiller University Jena Jena, Germany; University of Freiburg Freiburg; University of Oxford; Technische Universität Munchen - Technical University Munich - Université Technique de Munich (TUM); University Medical Center of the Johannes Gutenberg-University Mainz; Institute of Molecular Biology Mainz, Germany (IMB); Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU); Institute of Chemistry, The Hebrew University of Jerusalem; The Hebrew University of Jerusalem (HUJ); Centre de Biologie Structurale Montpellier (CBS); Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM); Laboratoire Interdisciplinaire de Physique Saint Martin d’Hères (LIPhy ); Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA); Universität Ulm - Ulm University Ulm, Allemagne; University of Illinois at Urbana-Champaign Urbana (UIUC); University of Illinois System; Ludwig-Maximilians University Munich (LMU); University of Warwick Coventry; Universiteit Leiden = Leiden University; University Hospital Düsseldorf; Delft University of Technology (TU Delft)
    • بيانات النشر:
      HAL CCSD
      Nature Publishing Group
    • الموضوع:
      2023
    • Collection:
      Université de Montpellier: HAL
    • نبذة مختصرة :
      International audience ; Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study of biomolecular structure and dynamics in vitro and in vivo. We performed an international blind study involving 19 laboratories to assess the uncertainty of FRET experiments for proteins with respect to the measured FRET efficiency histograms, determination of distances, and the detection and quantification of structural dynamics. Using two protein systems with distinct conformational changes and dynamics, we obtained an uncertainty of the FRET efficiency ≤0.06, corresponding to an interdye distance precision of ≤2 Å and accuracy of ≤5 Å. We further discuss the limits for detecting fluctuations in this distance range and how to identify dye perturbations. Our work demonstrates the ability of smFRET experiments to simultaneously measure distances and avoid the averaging of conformational dynamics for realistic protein systems, highlighting its importance in the expanding toolbox of integrative structural biology.
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/36973549; hal-04120543; https://hal.science/hal-04120543; https://hal.science/hal-04120543/document; https://hal.science/hal-04120543/file/2023_NatureMethods.pdf; PUBMED: 36973549; PUBMEDCENTRAL: PMC10089922
    • الرقم المعرف:
      10.1038/s41592-023-01807-0
    • Rights:
      http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess
    • الرقم المعرف:
      edsbas.A637BA72