Metal contamination and studies of copper-binding proteins from tilapia collected from Shing Mun River. ; Metal contamination & studies of copper-binding proteins from tilapia collected from Shing Mun River

Szeto Tsz Kwan Leo. ; Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. ; Includes bibliographical references (leaves 112-120). ; Abstracts in English and Chinese. ; Abstract --- p.i ; 摘要 --- p.iii ; Acknowledgements --- p.v ; Table of Contents --- p.vi ; List of Tables --- p.ix ; List of Figures --- p.x ; Abbreviations --- p.xii ; Chapter Chapter 1 --- Introduction --- p.1 ; Chapter 1.1 --- Heavy metals contaminations in Shing Mun River --- p.1 ; Chapter 1.1 --- Importance of copper regulation and role of liverin copper metabolism --- p.6 ; Chapter 1.1.1 --- Role of copper --- p.6 ; Chapter 1.1.2 --- Toxicity due to unbalanced copper regulation --- p.7 ; Chapter 1.1.3 --- Function of liver in copper detoxification --- p.9 ; Chapter 1.2 --- Aims and rationale of this research --- p.11 ; Chapter Chapter 2 --- Heavy metal concentrations of tilapia samples collected from Shing Mun River --- p.12 ; Chapter 2.1 --- Introduction --- p.12 ; Chapter 2.1.1 --- Sampling sites - Fo Tan and Siu Lek Yuen Nullah --- p.12 ; Chapter 2.1.2 --- Tilapia samples collected from the sites --- p.16 ; Chapter 2.1.3 --- Tilapia as a study model --- p.18 ; Chapter 2.1.4 --- Bioavailability of heavy metals in water --- p.19 ; Chapter 2.1.5 --- Metal content in liver --- p.20 ; Chapter 2.1.6 --- Aim of this chapter --- p.20 ; Chapter 2.2 --- Materials and Methods --- p.22 ; Chapter 2.2.1 --- Collection of control and field samples --- p.22 ; Chapter 2.2.2 --- Heavy metal concentrations determination --- p.23 ; Chapter 2.2.3 --- Homogenization of liver cells --- p.24 ; Chapter 2.2.4 --- Subcellular fractionation --- p.24 ; Chapter 2.2.5 --- Determination of copper and zinc content in each subcellular fraction --- p.253 ; Chapter 2.3 --- Results --- p.27 ; Chapter 2.3.1 --- Physical data --- p.27 ; Chapter 2.3.2 --- Metal concentrations in liver and muscle --- p.27 ; Chapter 2.3.3 --- Copper and zinc subcellular distribution in liver cell --- p.33 ; Chapter 2.4 --- Discussion --- p.36 ; Chapter 2.4.1 --- Difference in metal ...