Anticancer Activity of Bruceine A Isolated from The Seeds of Brucea javanica on Hela Cell

Brucea javanica seeds has been used traditionally to treat cancer, even though the scientific base of this has not been completely investigated. Bruceine A from Brucea javanica exhibit biological activities as a antimalarial, antitumor, antiviral and antiamoebic activities. This study is to examine the anticancer activity of Bruceine A from Brucea javanica seeds include cytotoxic activity and induced p53 protein expression on Hela cell. Cytotoxicity test of Bruceine A from Brucea javanica seeds has been performed by counting method of the cell directly with trypan blue exclusion dye. Analysis of induced p53 protein expression on Hela cell was detected by using immunocytochemistry technique. Cell that stained positive p53 protein showed that there was nucleus or cytoplasm stained with brown color, while the one stained negative p53 protein would be purple. From cytotoxicity test results of Bruceine A from Bruceine javanica seeds against Hela cell, value IC 50 obtained as big as 76.4 μg/ml. From observation of p53 protein expression on Hela cells by immunocytochemistry staining, it can be seen that Bruceine A from Brucea javanica seeds can increase the expression of p53 protein Hela cells by 44.3%. From the result of this study, it can be concluded that Bruceine A from Brucea javanica seeds have anticancer activity through inhibition mechanism of Hela cell proliferation, by activating the tumor suppressor gene such as p53.